All citybound lanes on the Monash Freeway were shut down after three cars and a rider collided about 6.15am today.
The 20-year-old female motorbike rider was rushed to hospital with life-threatening injuries but later died.
Who has right of way when a bus wants to pull out?
All drivers of the vehicles stopped at the scene.
Police said the drivers of all of the vehicles stopped at the scene.
The driver of a Nissan hatchback suffered minor injuries and was taken to hospital for treatment.
The driver of the Nissan Patrol, a 34-year-old Pakenham man, has this afternoon been charged with dangerous driving causing death.
He will appear before Melbourne Magistrates’ Court on June 24.
Commuters faced delays of up to 50 minutes during the morning peak hour.